An improved liquid nitrogen freezing method for establishing canine models of femoral head necrosis / 南方医科大学学报

<p><b>OBJECTIVE</b>To establish an canine model of femoral head osteonecrosis using an improved liquid nitrogen freezing method.</p><p><b>METHODS</b>Sixteen adult canines were divided into 4 groups at random and subjected to instant freezing of the unilateral femoral head with liquid nitrogen. The dogs were observed at 2, 4, 8, and 16 weeks after the operation for radiographic, gross and pathological changes of the femoral head.</p><p><b>RESULTS</b>Significant radiographic, gross and pathological changes occurred in the femoral head after the freezing.</p><p><b>CONCLUSION</b>Improved liquid nitrogen freezing of the femoral head provides a simple and convenient method for establishing animal models of femoral head osteonecrosis.</p>